Functional acetylcholine receptor--electroplax membrane microsacs (vesicles): purification and characterization.
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چکیده
منابع مشابه
Acetylcholine receptor: characterization of the voltage-dependent regulatory (inhibitory) site for acetylcholine in membrane vesicles from Torpedo californica electroplax.
Evidence for a voltage-dependent regulatory (inhibitory) site on the nicotinic acetylcholine receptor to which acetylcholine binds was obtained in membrane vesicles prepared from the Torpedo californica electric organ. Two rate coefficients, JA and alpha, which pertain to the receptor-controlled ion flux, were measured. A 1000-fold concentration range of acetylcholine was used in a transmembran...
متن کاملAcetylcholine-receptor-mediated ion flux in electroplax membrane microsacs (vesicles): change in mechanism produced by asymmetrical distribution of sodium and potassium ions.
The kinetics of acetylcholine-receptor-mediated efflux of inorganic ions from electroplax microsacs of Electrophorus electricus in the presence of varying alkali metal ion concentrations on both sides of the membrane have been investigated. The efflux, a monophasic process when the ion distribution is symmetrical (the same concentrations and types of ions on both sides of the membrane), becomes...
متن کاملReconstitution of functional membrane-bound acetylcholine receptor from isolated Torpedo californica receptor protein and electroplax lipids.
Purified acetylcholine receptor and total lipids, both extracted from Torpedo californica electroplax, were utilized to reconstitute chemically excitable membrane vesicles. Reconstitution was achieved by dialysis of the extraction detergent, octyl beta-D-glucoside from protein/lipid incubation mixtures. The reconstituted preparations could be fractionated by sucrose density gradient centrifuga...
متن کاملCharacterization and partial purification of the acetylcholine receptor from Torpedo electroplax.
Binding of acetylcholine in the concentration range 1 nM-1 muM was measured by equilibrium dialysis to a particulate preparation of Torpedo electroplax, without or with prior treatment of the tissue with one of three chemical modifying reagents. Significant reduction in binding of acetylcholine resulted after treatment with 1,4-dithiothreitol, p-chloromercuribenzoate, or p-(trimethylammonium)-b...
متن کاملAcetylcholine-receptor-mediated ion flux in electroplax membrane preparations.
The kinetics of acetylcholine-receptor-mediated sodium efflux from electroplax microsacs of electrophorus electricus has been analyzed. This led to the discovery that only a small fraction of the observed efflux is affected by chemical effectors such as carbamylcholine. Experimental conditions were chosen so that the receptor-mediated flux could be analyzed without the measurements' being obscu...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1977
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.74.2.482